Scientist, Molecular and Protein Engineering

About MeiraGTx

MeiraGTx is a clinical-stage gene therapy company focused on developing potentially curative treatments for patients living with serious diseases. We currently have six programs in clinical development including three ocular indications, a salivary gland condition, and a Parkinson’s disease program. Our initial focus on diseases of the eye, salivary gland and central nervous system is based on the significant unmet medical need coupled with the high potential gene therapy has to provide meaningful clinical benefit in these areas. With headquarters in New York and London, our global footprint provides us the opportunity to partner with leading institutions around the world, allowing us to deepen our understanding of diseases and their progression.

Our team was built with deep expertise in gene therapy development, allowing us to efficiently advance our programs from preclinical to clinical development. Our core capabilities in viral vector design and optimization and gene therapy manufacturing give us a differentiated approach to developing gene therapies. Additionally, we are developing proprietary technology to potentially enable innovative gene therapy treatments whose expression can be turned on and off with an easily administered small molecule. We believe temporal control of gene therapy products has the potential to transform the gene therapy landscape.

Our state-of-the-art manufacturing facility, completed in early 2018, was designed to meet global regulatory requirements, including the current good manufacturing practices (cGMP) required by the Medicines and Healthcare products Regulatory Agency (MHRA) in the UK and the U.S. Food and Drug Administration (FDA). The 29,000-square foot facility has the flexibility and capacity to produce sufficient product for all our clinical trials and will scale to commercial capacity.

We are seeking a motivated, enthusiastic, well-rounded Ph.D. level individual with in-depth knowledge of structural and computational biology, neuroscience, transcriptional regulation, virology, or AAV-based gene therapy methodologies to be a part of an early discovery biology group to develop better, smarter gene therapies.  For this position, the successful candidate will lead a small group of junior scientists to generate and experimentally validate innovative approaches to improving expression and potency of therapeutic gene targets in the MeiraGTx pipeline.

ROLES AND RESPONSIBILITIES

The scientist is responsible for:

  • In a collaborative environment, manage a small group of junior scientists with technical skills in molecular cloning, mammalian cell line culturing and transfections, in vitro biochemical/cellular assay development, and AAV vector production.
  • Use of structural biology and related computational tools (e.g. Schrodinger/Rosetta) to correlate macromolecular structure to function and design new transgenes with specific biological improvements.
  • Use of bioinformatics tools to come up with innovative solutions to improving transgene or transgene cassette properties.
  • Macromolecular quantification and characterization using techniques such as SDS-PAGE, western blotting, ELISA, qPCR, flow cytometry, and fluorescence microscopy.
  • Development of in vitro biochemical activity and cellular assays to assess engineered transgene function.

QUALIFICATIONS AND BACKGROUND REQUIREMENTS

Educational Requirements

  • Ph.D. or equivalent in a life science discipline and 0-5 years of post-doctoral work experience.

Desirable skills

  • Direct experience with the various structural biology methods such as protein crystallography, NMR spectroscopy, or cryo electron microscopy.
  • Direct experience with mining and extracting information from genomic databases such as transcriptional data from ENCODE and FANTOM consortiums.
  • Extensive experience with DNA cloning, gel electrophoresis, aseptic cell culturing, AAV/LV production and transduction, western blotting, qPCR, flow cytometry, fluorescence microscopy, ELISA, liquid chromatography, and biochemical/cellular assay development.
  • Familiarity of in vivo pharmacology is a plus.
  • Experience working in a gene therapy company with similar responsibilities is a plus.
  • Excellent oral and written communication skills
  • Demonstration of detailed record keeping and data documentation
  • Strong inter-personnel skills

TRAVEL

  • 0-15%

With a positive attitude, be prepared to learn, have fun, and make impactful contributions as part of an integral member of MeiraGTx!

Bioinformatician, Molecular and Protein Engineering

About MeiraGTx

MeiraGTx is a clinical-stage gene therapy company focused on developing potentially curative treatments for patients living with serious diseases. We currently have six programs in clinical development including three ocular indications, a salivary gland condition, and a Parkinson’s disease program. Our initial focus on diseases of the eye, salivary gland and central nervous system is based on the significant unmet medical need coupled with the high potential gene therapy has to provide meaningful clinical benefit in these areas. With headquarters in New York and London, our global footprint provides us the opportunity to partner with leading institutions around the world, allowing us to deepen our understanding of diseases and their progression.

Our team was built with deep expertise in gene therapy development, allowing us to efficiently advance our programs from preclinical to clinical development. Our core capabilities in viral vector design and optimization and gene therapy manufacturing give us a differentiated approach to developing gene therapies. Additionally, we are developing proprietary technology to potentially enable innovative gene therapy treatments whose expression can be turned on and off with an easily administered small molecule. We believe temporal control of gene therapy products has the potential to transform the gene therapy landscape.

Our state-of-the-art manufacturing facility, completed in early 2018, was designed to meet global regulatory requirements, including the current good manufacturing practices (cGMP) required by the Medicines and Healthcare products Regulatory Agency (MHRA) in the UK and the U.S. Food and Drug Administration (FDA). The 29,000-square foot facility has the flexibility and capacity to produce sufficient product for all our clinical trials and will scale to commercial capacity.

We are seeking a motivated, enthusiastic, well-rounded Ph.D. level individual with in-depth knowledge of structural and computational biology, neuroscience, transcriptional regulation, virology, or AAV-based gene therapy methodologies to be a part of an early discovery biology group to develop better, smarter gene therapies.  For this position, the successful candidate will lead a small group of junior scientists to generate and experimentally validate innovative approaches to improving expression and potency of therapeutic gene targets in the MeiraGTx pipeline.

ROLES AND RESPONSIBILITIES

The bioinformatician is responsible for:

• Designing, developing, and operating computational pipelines for the analysis of next-generation sequencing data.
• Evaluating the performance of novel technology platforms and assays
• Managing and archiving large data sets from next-generation sequencing experiments
• Working directly with biologists to analyze biomedical data and address scientific problems using bioinformatics and machine learning techniques
• Documenting and benchmarking established data analysis pipelines

QUALIFICATIONS AND BACKGROUND REQUIREMENTS

Educational Requirements

• M.S. or Ph.D. in Bioinformatics, Computer Science or related field required with 2+ years relevant experience applying quantitative approaches to solving biological problems

Desirable skills

• Demonstrated experience in the design, execution and interpretation of biological experiments generating large scale molecular datasets, especially RNA-seq, ATAC-Seq and other NGS data-types.
• Outstanding programming skills in bio-computational languages such as R and Python.
• Understanding of whole-genome or transcriptome alignment programs such as Bowtie, Trinity, RNA-STAR, Kallisto
• In depth knowledge of relevant public and proprietary databases, methods and tools.
• Ability to effectively interface with biologists to communicate/discuss results, hypotheses, and follow-up experiments
• Experience with implementing and deploying machine learning models is a plus
• Familiarity with cloud computing infrastructures on AWS and working with cloud-based tools is a plus
• Understanding of the biology of gene regulation or neuroscience is a plus

TRAVEL
• 0-15%

With a positive attitude, be prepared to learn, have fun, and make impactful contributions as part of an integral member of MeiraGTx!

Research Associate, Molecular and Protein Engineering

About MeiraGTx

MeiraGTx is a clinical-stage gene therapy company focused on developing potentially curative treatments for patients living with serious diseases. We currently have six programs in clinical development including three ocular indications, a salivary gland condition, and a Parkinson’s disease program. Our initial focus on diseases of the eye, salivary gland and central nervous system is based on the significant unmet medical need coupled with the high potential gene therapy has to provide meaningful clinical benefit in these areas. With headquarters in New York and London, our global footprint provides us the opportunity to partner with leading institutions around the world, allowing us to deepen our understanding of diseases and their progression.

Our team was built with deep expertise in gene therapy development, allowing us to efficiently advance our programs from preclinical to clinical development. Our core capabilities in viral vector design and optimization and gene therapy manufacturing give us a differentiated approach to developing gene therapies. Additionally, we are developing proprietary technology to potentially enable innovative gene therapy treatments whose expression can be turned on and off with an easily administered small molecule. We believe temporal control of gene therapy products has the potential to transform the gene therapy landscape.

Our state-of-the-art manufacturing facility, completed in early 2018, was designed to meet global regulatory requirements, including the current good manufacturing practices (cGMP) required by the Medicines and Healthcare products Regulatory Agency (MHRA) in the UK and the U.S. Food and Drug Administration (FDA). The 29,000-square foot facility has the flexibility and capacity to produce sufficient product for all our clinical trials and will scale to commercial capacity.

We are seeking a motivated, enthusiastic, well-rounded individual with experience in molecular biology and biochemistry to be a part of an early pre-clinical stage Molecular and Protein Engineering group to develop better, smarter gene therapies.  For this position, under the guidance of senior scientists, the successful candidate will be primarily responsible for the general laboratory upkeep of the research group and assisting in the construction of mammalian plasmids harboring engineered transgene cassettes, performing transient expressions of said plasmids in relevant mammalian cell lines, and subsequent testing of the expressed transgenes for improved functions using in vitro biochemical and/or cell-based assays.

ROLES AND RESPONSIBILITIES

The Research Associate is responsible for:

  • General laboratory maintenance and record keeping
  • Collaboration with current lab members
  • Maintenance of mammalian cell lines or primary cultures
  • Animal husbandry and handling including performing rodent injections and/or surgeries
  • Collection and histological analysis of tissue samples
  • Molecular cloning and sub-cloning using standard restriction enzyme-based and seamless DNA fragment based-assembly techniques
  • Write up concise research reports/presentations and provide periodic updates to the team.

QUALIFICATIONS AND BACKGROUND REQUIREMENTS

Educational Requirements

  • BS or equivalent in a life science discipline and 0-2 years of related lab experience.

Desirable skills

  • Excellent organizational and record keeping skills
  • Experience with mouse breeding, handling, tail vein and/or stereotactic injections strongly preferred

Technician, Molecular and Protein Engineering

About MeiraGTx

MeiraGTx is a clinical-stage gene therapy company focused on developing potentially curative treatments for patients living with serious diseases. We currently have six programs in clinical development including three ocular indications, a salivary gland condition, and a Parkinson’s disease program. Our initial focus on diseases of the eye, salivary gland and central nervous system is based on the significant unmet medical need coupled with the high potential gene therapy has to provide meaningful clinical benefit in these areas. With headquarters in New York and London, our global footprint provides us the opportunity to partner with leading institutions around the world, allowing us to deepen our understanding of diseases and their progression.

Our team was built with deep expertise in gene therapy development, allowing us to efficiently advance our programs from preclinical to clinical development. Our core capabilities in viral vector design and optimization and gene therapy manufacturing give us a differentiated approach to developing gene therapies. Additionally, we are developing proprietary technology to potentially enable innovative gene therapy treatments whose expression can be turned on and off with an easily administered small molecule. We believe temporal control of gene therapy products has the potential to transform the gene therapy landscape.

Our state-of-the-art manufacturing facility, completed in early 2018, was designed to meet global regulatory requirements, including the current good manufacturing practices (cGMP) required by the Medicines and Healthcare products Regulatory Agency (MHRA) in the UK and the U.S. Food and Drug Administration (FDA). The 29,000-square foot facility has the flexibility and capacity to produce sufficient product for all our clinical trials and will scale to commercial capacity.

We are seeking a motivated, enthusiastic, well-rounded individual with experience in molecular biology and biochemistry to be a part of an early pre-clinical stage Molecular and Protein Engineering group to develop better, smarter gene therapies.  For this position, under the guidance of senior scientists, the successful candidate will be primarily responsible for assisting in the construction of mammalian plasmids harboring engineered transgene cassettes, performing transient expressions of said plasmids in relevant mammalian cell lines, and subsequent testing of the expressed transgenes for improved functions using in vitro biochemical and/or cell-based assays.

ROLES AND RESPONSIBILITIES

The Technician is responsible for:

  • Restocking of common laboratory consumables.
  • Molecular cloning and sub-cloning using standard restriction enzyme-based and seamless DNA fragment based-assembly techniques.
  • Propagation and purification plasmids.
  • Maintenance of mammalian cell lines.
  • Transient expression of transgenes in mammalian cell lines.
  • Protein characterization using techniques such as SDS-PAGE, Western Blotting, plate reader based biochemical assays, and fluorescence microscopy cellular assays.
  • Write up concise research reports/presentations and provide periodic updates to the team.

QUALIFICATIONS AND BACKGROUND REQUIREMENTS

Educational Requirements

  • BS or equivalent in a life science discipline and 0-2 years of related lab experience.

Desirable skills

  • General biological lab experience
  • PCR and various cloning techniques
  • Aseptic techniques for handling bacterial and mammalian cell cultures
  • Bacterial transformations
  • Plasmid purification from bacterial overnight cultures
  • Familiarity with mammalian cell line culturing
  • Transient expression of plasmids in mammalian cell lines
  • Experience with protein purification a plus
  • Familiarity with plate-based colorimetric/fluorescent assays
  • Familiarity with fluorescent microscopy a plus
  • Familiarity with crystal protein structures a plus
  • Excellent oral and written communication skills
  • Demonstration of detailed record keeping and data documentation
  • Strong inter-personnel skills

TRAVEL

  • 0-5%

With a positive attitude, be prepared to learn, have fun, and make an impactful contribution as part of an integral member of the team!